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affinity biologicals/Fibrinogen Polyclonal Antibody - HRP Conjugated/SAFG-HRP

  • 产品编号: SAFG-HRP
  • 美  元  价: 待定
  • 市  场  价: ¥0.00
  • 品       牌:
  • 产       地: 美国(厂家直采)
  • 公       司: affinitybiologicals
  • 产品分类:
  • 公司分类: Antibodies
affinity biologicals/Fibrinogen Polyclonal Antibody - HRP Conjugated/SAFG-HRP
  • affinity biologicals/Fibrinogen Polyclonal Antibody - HRP Conjugated/SAFG-HRP
商品介绍

Description

Fibrinogen Polyclonal Antibody – HRP Conjugated

Affinity’s Fibrinogen Polyclonal Antibody – HRP Conjugated is the base level of our horseradish peroxidase conjugated Fibrinogen antibodies.  The purity of IgG is typically 90% and is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v).  The titre is essentially the same as the starting antiserum and each vial typically contains the amount of IgG recovered from one milliliter of antiserum however this IgG has been conjugated with Horseradish Peroxidase as an enzyme reporter.  This Fibrinogen Polyclonal Antibody – HRP Conjugated is generally intended for use as labeled primary antibodies in applications such as immunoassay and immunoblotting.


Product Code: SAFG-HRP

Retail Product Size: 0.2mg vial

Host Animal: Sheep Anti-Human Fibrinogen Polyclonal Antibody – HRP Conjugated

Species Cross Reactivity: View Chart

Product Datasheet: Fibrinogen Polyclonal Antibody hrp conjugated anti-human sheep IgG


Description of Fibrinogen (Fg)

Human fibrinogen is a 340 kDa plasma protein produced in the liver.  Plasma concentrations are typically 1.7 – 3.5 g/L (5-10 μM).  The intact fibrinogen molecule consists of two identical subunits, each consisting of three non-identical polypeptide chains denoted as Aα, Bβ and γ.  The letters A and B in the Aα and Bβ chains designate, respectively, fibrinopeptide A (FpA, residues 1-16), and fibrinopeptide B (FpB, residues 1-14), which are cleaved by thrombin upon conversion of fibrinogen to fibrin.  The fibrin monomers polymerize in a half-overlap fashion to form insoluble fibrin fibrils. The polymerised fibrin is subsequently stabilized by activated Factor XIII that forms amide linkages between γ chains and, to a lesser extent, α chains of the fibrin molecules.

Proteolysis of fibrinogen by plasmin initially liberates C-terminal residues from the Aα chain to produce fragment X (intact D-E-D, which is still clottable).  Fragment X is further degraded to non-clottable fragments Y (D-E) and D.  Fragment Y can be digested into its constituent D and E fragments.  Proteolysis of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the γ chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD.  The molecular weights of the cleavage fragments produced from human crosslinked fibrin are: 184 kDa for fragment DD, 92 kDa for D, 50 kDa for E, 1.54 kDa for FpA and 1.57 kDa for FpB.

Most of the fibrinogen in the circulation consists of 2 copies of each chain (Aα2, Bβ2, γA2), but in normal plasma approximately 10% of the fibrinogen molecules contain one γA chain and one variant γ chain (termed γ), in which the c-terminal AGDV residues are replaced with the amino acid sequence VRPEHPAETEYDSLYPEDDL.  This variant fibrinogen is commonly referred to as fibrinogen gamma prime (γA) but has also been called fibrinogen 2 or peak 2 fibrinogen because it elutes separately from fibrinogen 1 (γA2) by ion exchange chromatography.  Residues 414-427 of the γ chain of fibrin gamma prime (contain a high-affinity binding site for exosite II of thrombin, which allows the active site of bound thrombin to remain available to interact with substrates while demonstrating resistance to heparin mediated inhibition by antithrombin1-4.

References and Reviews

  1. Hantgan RR, Francis CW, Marder VJ; Fibrinogen Structure and Physiology; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 277-300, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
  2. Binnie CG, Lord ST; The Fibrinogen Sequences that Interact with Thrombin;  Blood 81,  pp 3186-3192, 1993.
  3. Pospisil CH, Stafford AR, Fredenburgh JC, Weitz JI; Evidence that both Exosites on Thrombin Participate in Its High Affinity Interaction with Fibrin; JBC 278, pp 21584-21591, 2003.
  4. Medved L, Weisel JW; Recommendations for Nomenclature on Fibrinogen and Fibrin; JTH 7, pp 355-359, 2009.
品牌介绍

Affinity Biologicals,Inc.是全球范围内用于血栓形成和止血研究与诊断的产品的主要制造商。我们的产品包括广泛的抗体,结合物,用于人类和动物分析物免疫测定的抗体对,完整的ELISA试剂盒的VisuLize系列以及包括VisuDep免疫缺陷因子缺乏血浆,VisuCal校准血浆的全系列人类血浆产品和VisuCon控制血浆。服务包括蛋白质纯化,抗体生产,测定开发,定制制造(包括定制冻干和能力验证材料)。


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Affinity Biologicals,Inc.是全球范围内用于血栓形成和止血研究与诊断的产品的主要制造商。我们的产品包括广泛的抗体,结合物,用于人类和动物分析物免疫测定的抗体对,完整的ELISA试剂盒的VisuLize系列以及包括VisuDep免疫缺陷因子缺乏血浆,VisuCal校准血浆的全系列人类血浆产品和VisuCon控制血浆。服务包括蛋白质纯化,抗体生产,测定开发,定制制造(包括定制冻干和能力验证材料)。

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