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affinity biologicals/Apolipoprotein-H Paired Antibody Set/APOH-EIA

  • 产品编号: APOH-EIA
  • 美  元  价: 待定
  • 市  场  价: ¥0.00
  • 品       牌:
  • 产       地: 美国(厂家直采)
  • 公       司: affinitybiologicals
  • 产品分类:
  • 公司分类: Paired Antibody Sets
affinity biologicals/Apolipoprotein-H Paired Antibody Set/APOH-EIA
  • affinity biologicals/Apolipoprotein-H Paired Antibody Set/APOH-EIA
商品介绍

Description

Apolipoprotein-H Paired Antibody Set

Affinity’s Apolipoprotein-H Paired Antibody Set consists of matched capture and detecting antibodies that have been titrated and optimized for use in sandwich style ELISA assays. The product as provided contains sufficient capture and detecting antibodies for five full 96-well microplates and contains a detailed protocol sheet containing directions for use, recipes for solutions and sources for additional materials required. This Apolipoprotein-H Paired Antibody Set is intended to facilitate the end user in establishing an “in-house” immunoassay for research purposes only and must not be used for diagnostic applications.  Assay validation is the responsibility of the end user.


Product Code: APOH-EIA

Supplied Materials:

  1. Capture Antibody (APOH-EIA-C): One yellow-capped vial containing 0.4 ml of polyclonal affinity purified anti-B2GP1 antibody for coating plates.
  2. Detecting Antibody (APOH-EIA-D):  One red-capped tube containing 0.4 ml of peroxidase conjugated affinity-purified polyclonal anti-B2GP1 antibody for detection of captured B2GP1.

Related Products:  VisuLize Buffer Pak APOH Deficient Plasma

Species Cross Reactivity: View Chart

Product Datasheet: APOH Apolipoprotein-H Beta-2-Glycoprotein-2 Antigen Matched Pair Antibody Set for ELISA - APOH-EIA


Description of Beta-2-Glycoprotein-1 (Apolipoprotein-H, APOH)

Beta-2-Glycoprotein 1 (B2GP1), also known as Apolipoprotein-H (APOH), is a plasma glycoprotein that circulates at a concentration of 200 ug/ml (4 μM). Synthesized in the liver, β2GP1 is a single chain molecule of 48 kDa, consisting of five repeating internally disulphide-bonded structures referred to as sushi domains. Relative to other glycoproteins, β2GP1 has an unusually high content of cysteine (6.2%), proline (8.3%) and carbohydrate (19%). Almost half the circulating β2GP1 in plasma is associated with lipoproteins of all major fractions. β2GP1 has been demonstrated to bind negatively charged phospholipids, heparin and platelets. Although the precise function(s) are as yet unknown, β2GP1 has been demonstrated to interfere with blood coagulation by competitively binding to negatively charged phospholipid surfaces exposed during cell activation or damage. Recent evidence also implicates β2GP1 as a cofactor recognized by anti-phospholipid antibodies present in some autoimmune disorders such as systemic lupus erythematosus (SLE)1-3.

References and Review

  1. Lee, NS, Brewer HB Jr., Osborne JC Jr.; β2Glycoprotein I: Molecular Properties of an Unusual Apolipoprotein, Apolipoprotein H; JBC 258, pp 4765-4770, 1983.
  2. Schousboe I; β2Glycoprotein I: A Plasma Inhibitor of the Contact Activation of the Intrinsic Blood Coagulation Pathway. Blood 66, pp 1086-1091, 1985.
  3. Nimpf J, Bevers EM, Bomans PHH, Till U, Wurm H, Kostner GM, Zwaal RFA; Prothrombinase activity of human platelets is inhibited by β2Glycoprotein I; Biochimica et Biophysica Acta 884, pp 142-149, 1986.
品牌介绍

Affinity Biologicals,Inc.是全球范围内用于血栓形成和止血研究与诊断的产品的主要制造商。我们的产品包括广泛的抗体,结合物,用于人类和动物分析物免疫测定的抗体对,完整的ELISA试剂盒的VisuLize系列以及包括VisuDep免疫缺陷因子缺乏血浆,VisuCal校准血浆的全系列人类血浆产品和VisuCon控制血浆。服务包括蛋白质纯化,抗体生产,测定开发,定制制造(包括定制冻干和能力验证材料)。


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affinity biologicals定制多克隆抗体生产

Affinity Biologicals,Inc.在制造,纯化和标记抗体,纯化蛋白质以及开发检测方法方面拥有25年以上的经验。抗体是在省级许可的研究机构生产的。以下价格以美元计:


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定制多克隆抗体生产的标准包装包括110天的免疫时间表。包括所有动物费用,维护和技术费用。客户提供用于免疫和测试的抗原。完成时的典型抗血清产量为每只绵羊700毫升或每只山羊500毫升。将叠氮化钠添加到抗血清中以防止微生物生长。


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