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affinity biologicals/Prothrombin - Factor II Paired Antibody Set/FII-EIA

  • 产品编号: FII-EIA
  • 美  元  价: 待定
  • 市  场  价: ¥0.00
  • 品       牌:
  • 产       地: 美国(厂家直采)
  • 公       司: affinitybiologicals
  • 产品分类:
  • 公司分类: Paired Antibody Sets
affinity biologicals/Prothrombin - Factor II Paired Antibody Set/FII-EIA
  • affinity biologicals/Prothrombin - Factor II Paired Antibody Set/FII-EIA
商品介绍

Description

Prothrombin – Factor II Paired Antibody Set

Affinity’s Prothrombin – Factor II Paired Antibody Set consists of matched capture and detecting antibodies that have been titrated and optimized for use in sandwich style ELISA assays. The product as provided contains sufficient capture and detecting antibodies for five full 96-well microplates and contains a detailed protocol sheet containing directions for use, recipes for solutions and sources for additional materials required. This Prothrombin – Factor II Paired Antibody Set is intended to facilitate the end user in establishing an “in-house” immunoassay for research purposes only and must not be used for diagnostic applications.  Assay validation is the responsibility of the end user.


Product Code: FII-EIA

Supplied Materials:

  1. Capture Antibody (FII-EIA-C): One yellow-capped vial containing 0.5 ml of polyclonal affinity purified anti-Prothrombin antibody for coating plates.
  2. Detecting Antibody (FII-EIA-D):  One red-capped tube containing 0.5 ml of peroxidase conjugated affinity-purified polyclonal anti-Prothrombin antibody for detection of captured Prothrombin – Factor II.

Related Products:  VisuLize Buffer Pak VisuCal Antigen Calibrator Plasma

Species Cross Reactivity: View Chart

Product Datasheet: Factor II (F2 FII) Prothrombin Antigen Matched Pair Antibody Set for ELISA - FII-EIA


Description of Prothrombin – Factor II

Prothrombin (factor II, F.II) is a vitamin K-dependent glycoprotein produced in the liver.  The concentration of prothrombin in plasma is ~100 μg/ml (~1.4 μM).  Prothrombin is a single chain molecule with a molecular weight of 72 kDa.  Prothrombin consists of a catalytic domain followed by two kringle structures and an amino-terminal domain containing 10 γ-carboxy-glutamic acid (gla) residues. These gla residues allow prothrombin to bind to membranes that contain acidic phospholipids in a calcium dependent manner.  The binding to membranes is required for effective presentation of prothrombin as a substrate for activation by the prothrombinase complex, which consists of activated factor X, activated cofactor V and calcium on phospholipid membrane.  Activation by prothrombinase occurs by sequential cleavage after residue Arg320 then after Arg271 to produce the active protease α-thrombin (37 kDa) and the by-product prothrombin fragment 1.2 (35 kDa).  The product thrombin further cleaves prothrombin fragment 1.2 after residue Arg155 into individual prothrombin fragments 1 and 2.  The activity of α-thrombin in plasma is inhibited primarily by antithrombin and the rate of inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin.  Other physiological inhibitors of thrombin in the absence of heparin include α2macroglobulin and α1antitrypsin1-3.

References and Reviews

  1. Mann KG; Prothrombin and Thrombin; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 184-199, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
  2. Mann KG; Prothrombin; Methods in Enzymology 45, pp 123-156, 1976.
  3. Downing MW, Bloom JW, Mann KG; Comparison of the Inhibition of Thrombin by Three Plasma Protease Inhibitors; Biochemistry 17, pp 2649-2653, 1978.
品牌介绍

Affinity Biologicals,Inc.是全球范围内用于血栓形成和止血研究与诊断的产品的主要制造商。我们的产品包括广泛的抗体,结合物,用于人类和动物分析物免疫测定的抗体对,完整的ELISA试剂盒的VisuLize系列以及包括VisuDep免疫缺陷因子缺乏血浆,VisuCal校准血浆的全系列人类血浆产品和VisuCon控制血浆。服务包括蛋白质纯化,抗体生产,测定开发,定制制造(包括定制冻干和能力验证材料)。


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affinity biologicals的凝血–止血ELISA试剂盒

VisuLize Antigen试剂盒是凝血-止血ELISA试剂盒系列,用于定量测定VII因子(F7),VIII因子(F8),IX因子(F9),X因子(FX),XI因子(F11)和TAFI抗原使用双抗体酶联免疫吸附试验(ELISA)在人血浆样品中进行检测。


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